Rapp OptoElectronic GmbH | DF-Scope - Multi-Photon Imaging Package for Olympus BX51WI

A hallmark of multi-photon imaging is that all of the light emitted by the sample is known to emit from the focal volume as a result of the non-linear excitation of the fluorophore. High-sensitivity photomultiplier tubes (PMTs) are used to collect as many photons as possible in order to reconstruct the scanned image. The DF-Scope design allows for two detector paths to gather more emitted light – one above the sample and one below. If using a thin sample, like a brain slice, we recommend the lower (sub-stage) detectors for increased signal detection. Additional signal will be available at the trans detector path. This substage detector assembly is designed to work with a variety of Olympus condensers.

NOTE: The DF-Scope design requires an Olympus BX51WI and the following Olympus parts: WI-ARMAD, 5-UR710LP, and U-M619.

Features:

• Converts a standard Olympus BX51WI into a two-photon microscope while retaining standard microscope functions (transmitted light and epifluorescent imaging).
• Includes detector(s) and “whisperquiet” resonant scan box developed for the MOM™ Two-Photon microscope
• Upper and lower photodetectors for increased collection efficiency
• Fully compatible with the Sutter MPC-78 Large Moving Stage Platform and motorized focus drive
• Designed to be controlled with the Sutter MCS (MOM Computer System) Imaging Software including resonant scanning with MScan 2.0
• Also fully compatible with most multi-photon freeware such as ScanImage 5.0, Helioscan, and MPScope
• Breadboard format in scan pathway allows easy addition of photostimulation light sources to the main scanned laser path

Applications:

• In vivo and in vitro two-photon imaging
• Whole-cell imaging
• Intracellular imaging