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Etaluma | Imaging system LS820 Microscope

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Etaluma, Inc.

4360 Viewridge Ave, Suite B
San Diego, CA 92123

United States of America

Phone: (760) 281-2212

Mail: sales@etaluma.com
Web: https://etaluma.com/

Details

Etaluma | Imaging system LS820 Microscope

Blue, Green, and Red Fluorescence with Autofocus and Z-Stacking for Time Lapse Automation

The LS820 Microscope, a potent and innovative model, represents the cutting-edge in our fully automated three-channel motorized Z series. It incorporates the latest advancements in optics, camera technology, and user adaptability. Surpassing the manual LS620 model, the LS820 excels in image quality, Z-range, illumination, and software flexibility. Its motorized focus enables precise autofocus and z-stacks with 100 nm steps over a range exceeding 14 mm. The user-friendly software, easily configurable to generate readable and editable scripts, streamlines your automated microscopy experiments.

Features and Benefits

Images, Z-stacks, time-lapse series, and videos recorded directly to your computer
Bright field and phase contrast transmitted illumination
LED excitation and high performance optics provide near diffraction-limited resolution
Multi-OS software runs on Windows, Mac and Linux
Open deck allows microplates, microfluidic chips, slides, dishes, flasks, and deck-top chambers
Long range motorized Z for autofocus and Z-stacks down to 100 nm
Compact and robust design enables use inside cell culture hoods, incubators, hypoxic chambers, and on the bench
Detects blue, green and red fluorophores, including common probes such as Hoechst, DAPI, FITC, Fluo-4, GFP, Texas Red & mCherry
Open source Python control code available
Objective compatibility with standard lenses

Similar to all Etaluma microscopes, the LS820 employs a Semrock Brightline® Pinkel filter set tailored for our 405 nm, 488 nm, and 590 nm LED light sources. This comprehensive 5-filter set includes three excitation filters, one triple-band dichroic, and one triple-band emission filter. The LS820 employs a 12-bit monochrome CMOS sensor.

The new LS800 series microscopes are equipped with a low profile, multimodal illuminator that was developed in collaboration with Cairn Research Ltd (based on Aura/Aura Pro™ phase contrast illuminator, based on work by Dr Kevin Webb, Optics & Photonics Research Group, University of Nottingham: Webb, Kevin F. “Condenser‐free contrast methods for transmitted‐light microscopy.” Journal of microscopy 257.1 (2015): 8-22.

Excitation and Emission Profiles of LS820 filters

LS820 Microscope Specifications
LED Excitation:	405 nm, 488 nm, and 589 nm
Fluorescence Filters:	Blue: Excitation 370-410 nm, Emission 429-462 nm Green: Excitation 473-491 nm, Emission 502-561 nm Red: Excitation 580-598 nm, Emission 612-680 nm
Transmitted Modes:	Bright Field, Phase Contrast
Objectives:	1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil)
Camera:	High Sensitivity Monochrome CMOS BSI Sensor; 5 MP 12-Bit
Image Formats:	JPG, BMP, TIF, or PNG
Image Size:	Adjustable up to 2100 x 2100 pixels
Video Rates:	25 FPS (exposure limited)
Motorized Z:	14mm travel, 100 nm step, image-based autofocus, Z-stacks
Control Software:	LumaviewPro, multi operating system application
Computer Requirements:	Windows 10, 11; Core i7, 512TB SSD, 8GB RAM MacOS with M1; 512TB SSD, 8GB RAM
Linux Debian Distributions:	512TB SSD, 8GB RAM
Power Requirements:	80-264 V, 50-60 Hz 10W typical, 40W Max
Integration:	Python source under the MIT Open Source License
Dimensions:	22 cm x 14 cm x 11 cm
Weight:	5 Kg/11 lbs
Operating Conditions:	0°C - 42°C, 5% - 95% RH non-condensing

LumaviewPro application runs on
Windows, Mac, and Linux

Configurations (0)

For this microscope, no configurations are currently set up. Please contact the manufacturer or a specialist retailer of your choice for advice on your application.