Blue, Green, and Red Fluorescence with Autofocus and Z-Stacking for Time Lapse Automation

The LS820 Microscope, a potent and innovative model, represents the cutting-edge in our fully automated three-channel motorized Z series. It incorporates the latest advancements in optics, camera technology, and user adaptability. Surpassing the manual LS620 model, the LS820 excels in image quality, Z-range, illumination, and software flexibility. Its motorized focus enables precise autofocus and z-stacks with 100 nm steps over a range exceeding 14 mm. The user-friendly software, easily configurable to generate readable and editable scripts, streamlines your automated microscopy experiments.

Features and Benefits

• Images, Z-stacks, time-lapse series, and videos recorded directly to your computer
• Bright field and phase contrast transmitted illumination
• LED excitation and high performance optics provide near diffraction-limited resolution
• Multi-OS software runs on Windows, Mac and Linux
• Open deck allows microplates, microfluidic chips, slides, dishes, flasks, and deck-top chambers
• Long range motorized Z for autofocus and Z-stacks down to 100 nm
• Compact and robust design enables use inside cell culture hoods, incubators, hypoxic chambers, and on the bench
• Detects blue, green and red fluorophores, including common probes such as Hoechst, DAPI, FITC, Fluo-4, GFP, Texas Red & mCherry
• Open source Python control code available
• Objective compatibility with standard lenses

Similar to all Etaluma microscopes, the LS820 employs a Semrock Brightline® Pinkel filter set tailored for our 405 nm, 488 nm, and 590 nm LED light sources. This comprehensive 5-filter set includes three excitation filters, one triple-band dichroic, and one triple-band emission filter. The LS820 employs a 12-bit monochrome CMOS sensor.

The new LS800 series microscopes are equipped with a low profile, multimodal illuminator that was developed in collaboration with Cairn Research Ltd (based on Aura/Aura Pro™ phase contrast illuminator, based on work by Dr Kevin Webb, Optics & Photonics Research Group, University of Nottingham: Webb, Kevin F. “Condenser‐free contrast methods for transmitted‐light microscopy.” Journal of microscopy 257.1 (2015): 8-22.

Excitation and Emission Profiles of LS820 filters

LumaviewPro application runs on
Windows, Mac, and Linux