Etaluma | Imaging System LS850 Microscope

Blue, Green, and Red Fluorescence and Walk-Away Automation

The powerful LS850 Microscope represents the latest generation of our fully automated three-channel flagship model. It introduces advancements in optics, cameras, throughput, and user flexibility. The LS850 enhances image quality, motion speed, illumination, and software flexibility over the previous LS720 model. Notable features include exquisite XY motion control, motorized focus enabling autofocus and Z-stacks, and easy-to-configure software for seamless automated microscopy experiments.

The LS850 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long-term imaging.

Whether imaging multiple fields in your flasks or 1536 wells of cells with three fluorophores in a multi-day time-lapse, the LS850 opens up a new realm of walk-away automated microscopy!

Excitation and Emission Profiles of LS850 filters

Features and Benefits

• Images, tiled images, Z-stacks, time-lapse series, and videos recorded directly to your computer
• Bright field and phase-contrast transmitted illumination
• LED excitation and high-performance optics providing near-diffraction-limited resolution
• Multi-OS software for set-up and control across various locations
• High-speed stage with single-digit micron precision
• Long-range motorized Z for autofocus and Z-stacks down to 100 nm
• Compact and robust design suitable for use inside cell culture hoods, incubators, hypoxic chambers, and gloveboxes
• Detects blue, green, and red fluorophores, including common probes such as Hoechst, DAPI, FITC, Fluo-4, GFP, Texas Red & mCherry GFP
• Open-source Python control code available
• Objective compatibility with standard lenses and available with an optional automated 4-objective turret

LS850 Microscope Specifications

LED Excitation: 405 nm, 488 nm, and 589 nm

Fluorescence Filters:

• Blue: Excitation 370-410 nm, Emission 429-462 nm
• Green: Excitation 473-491 nm, Emission 502-561 nm
• Red: Excitation 580-598 nm, Emission 612-680 nm
  
  
• Transmitted Modes: Bright Field, Phase Contrast
• Objectives: 1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil)
• Camera: High Sensitivity Monochrome CMOS BSI Sensor; 5 MP 12-Bit
• Image Formats: JPG, BMP, TIF, or PNG
• Image Size: Adjustable up to 2100 x 2100 pixels
• Video Rates: 25 FPS (exposure limited)
• Automated XY Stage: 120 mm x 80mm travel, 1-3 micron positional repeatability
• Throughput: 96-well, 3 channel fluorescence, w/o autofocus - 6 minutes 30 seconds
• Motorized Z: 14mm travel, 100 nm step, image-based autofocus, Z-stacks
• Control Software: LumaviewPro, multi operating system application
• Computer Requirements: Windows 10, 11; Core i7, 512TB SSD, 8GB RAM
• MacOS with M1; 512TB SSD, 8GB RAM
• Linux Debian Distributions: 512TB SSD, 8GB RAM
• Power Requirements: 80-264 V, 50-60 Hz 10W typical, 40W Max
• Integration: Python source under the MIT Open Source License
• Dimensions: 39.8 cm W x 29.7 cm D x 29.7 cm H [15.6 in W x 11.6 in D x 11.6 in H]
• Weight: 10 Kg/22lbs
• Operating Conditions: 0°C - 42°C, 5% - 95% RH non-condensing

LumaviewPro application runs on Windows, Mac and Linux